Exploring the Regulatory Role of XIST-microRNAs/mRNA Network in Circulating CD4+ T Cells of Hepatocellular Carcinoma Patients

Hepatocellular carcinoma (HCC) is one of the most common cancers and main cause cancer-related death globally. Immune dysregulation CD4+ T cells has been identified to play a role in development HCC. Nevertheless, underlying molecular pathways HCC are not completely known. Thus, better understanding lncRNA-miRNA/mRNA network may yield novel insights into etiology or progression In this study, circulating were isolated from whole blood 10 healthy controls patients for next-generation sequencing expression lncRNAs, miRNAs, mRNAs. Our data showed that there different expressions 34 transcripts (2 XISTs, MIR222HGs; 29 mRNAs; 3 other types RNA) 13 miRNAs patients. The lncRNA-XIST-related their target mRNAs was confirmed using real-time quantitative polymerase chain reaction (qPCR) on samples 100 60 lncRNA–miRNA/mRNA regulation created interaction generated ENCORI revealed positive correlations infiltration total cells, particularly resting memory negative Th1 cells.